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Fractional synthesis rate was determined with the use of muscle biopsy samples taken from participants with postabsorptive status (basal period) and 1 and 3 h from EAA ingestion (treatment period). EAA and FSH were measured by radioimmunoassay in blood samples collected 2–3 h following EAA ingestion and measured by using the assay kit of Life Technologies (Athol, France). Statistical analysis Two-way analysis of variance was used to analyze the data. The Pearson correlation coefficient was used to assess differences between experimental groups. Multiple comparison and t-test was used to compare the change in testicular hormones during EAA and FSH administrations. The P values of significance were defined as p<0.05. EAA treatment The EAA treatment started at 4.00 pm but only on the third day (day 4) in order to minimize any side effects related to long-term use of an orally administered anabolic/androgenic steroid. The subjects consumed their meals and the meals were provided in the morning via a feeding tube with food and water with the exception of the lunch, the evening, or the nighttime; however, the subjects had unlimited access to water during the intake week. On the fourth day EAA intake was increased in the presence of protein, fat, lipids and carbohydrates, except that all meals were provided with carbohydrate in the form of sucrose, potato flakes, chocolate, and energy bars (Diet Coke or Gatorade) or without carbohydrate (Caffeinated tea, caffeine-free coffee, or tea with energy). At this time EAA was administered in a two-ml syringe using a double-blind design. No additional nutrients were given at these meals and participants did not receive any supplemental protein or vitamins or minerals (e.g. calcium). In addition to the diet and supplements EAA and fSH were provided through oral gavage (25 mg/kg) at the same time. The doses of nutrients were administered per week for 5 weeks with 1 week between bouts of EAA administration and 5 weeks between bouts of fSH administration; however, because the subjects were not familiar with doses and dose-dependence between oral gavage and feeding tube administration the doses were not adjusted according to the amount of EAA (EAA and fSH were administered to the same body weight but the amount of EAA administered was adjusted to match each trial). The average volume of feeding tube was 12 cm. The mean EAA (mmol/L) was 4.5 +/- 0.5, which is similar to the value found for insulin doses during the fasting or hyperinsul Similar articles: